Julington Creek is a stream in Duval County, Florida, near Jacksonville. Durbin Creek is a tributary. Julington Creek feeds into the St Johns River. Bordering the Julington-Durbin Preserve is a 2,031-acre nature preserve operated by the St. Johns River Water Management District in Duval County, Florida. Julington Creek is on the north side of the preserve, and Durbin Creek is to the south. The preserve has three designated hiking trails. Recreational activities include picnicking, horseback riding, bicycling, and hiking.
Harriet Beecher Stowe wrote about her visits to the creek while she was living in Mandarin, Florida, in Palmetto Leaves (1873).